PIB2 / YGL023C Overview
- Standard Name
- PIB2 4
- Systematic Name
- YGL023C
- SGD ID
- SGD:S000002991
- Feature Type
- ORF , Verified
- Description
- Phosphatidylinositol 3-phosphate binding protein; forms a distinct complex with TORC1, induced by glutamine in a dose-dependent manner, resulting in TORC1 activation, suggesting dual roles as both a glutamine sensor and a direct TORC1 activator; functions as a cysteine sensor involved in TORC1 activation; involved in leucine- and glutamine-responsive TORC1 activation, independent of the Gtr/Ego pathway; related to human PLEKHF1; contains a PI3P binding FYVE domain; similar to Fab1p and Vps27p 1 2 3 5 6
- Name Description
- PtdIns(3)p-Binding 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PIB2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phosphatidylinositol-3-phosphate binding protein that activates TORC1 signaling; localizes to vacuolar membrane and mitochondria
View computational annotations
Molecular Function
- Manually Curated
- enables amino acid sensor activity (IDA)
- enables L-cysteine binding (IMP, IDA)
- enables phosphatidylinositol-3-phosphate binding (IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in positive regulation of TORC1 signaling (IGI, IMP, IDA)
Cellular Component
- Manually Curated
- is active in fungal-type vacuole membrane (IDA)
- High-Throughput
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- PIB2/YGL023C is a non-essential gene in reference strain S288C, deletion mutants are viable but show reduced vegetative and competitive growth, including poorer anaerobic growth and sensitivity to hydrostatic pressure and oxidative stress (paraquat). pib2Δ alters metabolism and protein homeostasis, with increased alanine/valine (and glutamine under high pressure), increased glycogen, decreased serine and H2S (with cysteine), and impaired protein modification/redistribution under nitrogen starvation and cycloheximide. The mutant is broadly drug-sensitive, notably to caffeine, alcohols (ethanol, isobutanol, propanol), myriocin, aflatoxin B1, doxorubicin, diethyl phthalate, and rapamycin/sirolimus (with FYVE and conserved residue mutants also sirolimus‑sensitive), and shows reduced killer toxin resistance; resistance to wortmannin is unaffected. Conversely, pib2Δ increases resistance to Aβ42 (with reduced toxin‑induced cell death), tacrolimus, tunicamycin, DTT, poacic acid, polygodial, and several azole fungicides. Overexpression further decreases growth and tolerance to caffeine and sirolimus and promotes invasive growth.
Classical Genetics
- reduction of function
- null
- cell death: decreased rate
- chemical compound accumulation: increased
- growth in exponential phase: decreased
- hydrostatic pressure resistance: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- overexpression
- null
- anaerobic growth: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- killer toxin resistance: decreased
- oxidative stress resistance: decreased
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- toxin resistance: increased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
445 total interactions for 331 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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