TSA2 / YDR453C Overview
- Standard Name
- TSA2 1
- Systematic Name
- YDR453C
- SGD ID
- SGD:S000002861
- Aliases
- cTPxII 2
- Feature Type
- ORF , Verified
- EC Number
- 1.11.1.24
- Description
- Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication 1 2 3 4 5 6
- Name Description
- Thiol-Specific Antioxidant 7
- Paralog
- TSA1 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Tsa2p is 196 amino acids long, low in abundance and short-lived; abundance increases in response to DNA replication stress
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TSA2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic peroxidase involved in oxidative stress response, protein folding, and cell redox homeostasis
View computational annotations
Molecular Function
- Manually Curated
- enables peroxiredoxin activity (IDA)
Biological Process
- Manually Curated
- involved in cell redox homeostasis (IMP, IDA)
- involved in cellular response to heat (IDA)
- involved in cellular response to oxidative stress (IGI, IMP)
- involved in protein folding (IDA)
- involved in protein heterooligomerization (IPI)
- involved in protein homooligomerization (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant has increased G1 phase duration, increased reactive oxygen species and malonaldehyde accumulation, decreased anaerobic growth, decreased exponential phase growth rate, decreased oxidative stress resistance, decreased utilization of multiple carbon sources including galactose and glucose and nitrogen sources including glutamate and proline, abnormal vacuolar morphology, increased resistance to benzo[a]pyrene and toxaphene, and decreased competitive fitness; overexpression causes increased innate thermotolerance and increased vegetative growth; in large-scale studies, is haploinsufficient; the null mutant of paralog tsa1 is viable; the tsa2 tsa1 double mutant displays a synthetic growth defect
Classical Genetics
- null
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Tsa2p interacts physically with proteins involved in response to chemical; TSA2 interacts genetically with genes involved in response to chemical
107 total interactions for 72 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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