GNP1 / YDR508C Overview
- Standard Name
- GNP1 1
- Systematic Name
- YDR508C
- SGD ID
- SGD:S000002916
- Feature Type
- ORF , Verified
- Description
- Broad specificity amino acid permease; high-affinity glutamine permease; major serine permease with minor contributions from paralog Agp1p; role in serine uptake impacts cellular sphingolipid homeostasis; also transports Leu, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids 1 2 3 4 5
- Name Description
- GlutamiNe Permease 1
- Paralog
- AGP1 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Gnp1p is 663 amino acids long, moderate in abundance and very short-lived; palmitoylated on C663, phosphorylated on 23 residues, and ubiquitinylated on 13 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GNP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Amino acid transmembrane transporter that localizes to endoplasmic reticulum, mitochondria, cell periphery, and bud neck
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in amino acid transport (IDA)
- involved in intracellular sphingolipid homeostasis (IMP)
- involved in serine import across plasma membrane (IMP, IGI)
- involved in transmembrane transport (IDA)
Cellular Component
- High-Throughput
- located in cell periphery (HDA)
- located in cellular bud neck (HDA)
- located in endoplasmic reticulum (HDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant has impaired serine and glycine uptake, altered sphingolipid metabolism with decreased phytosphingosine levels and reduced ergosterol biosynthesis enzyme expression; increased resistance to sphingolipid compounds and rapamycin but decreased resistance to myriocin, cordycepin, cadmium, and antimicrobial peptides; decreased competitive fitness in minimal medium but increased fitness in synthetic complete and ethanol media; decreased chronological lifespan, oxidative stress resistance, and arginine utilization; moderate vacuolar fragmentation defects; overexpression decreases growth rate but increases UV resistance; encodes a glutamine permease that also transports serine and glycine; the null mutant of paralog agp1 is viable; the gnp1 agp1 double mutant displays a synthetic growth defect
Classical Genetics
- null
- unspecified
- gain of function
- null
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Gnp1p interacts physically with proteins involved in RNA catabolism; GNP1 interacts genetically with genes involved in response to chemical
318 total interactions for 251 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
Loading literature…