SSA2 / YLL024C Overview
- Standard Name
- SSA2 1
- Systematic Name
- YLL024C
- SGD ID
- SGD:S000003947
- Aliases
- YG102 1
- Feature Type
- ORF , Verified
- Description
- HSP70 family ATP-binding protein; role in protein folding and vacuolar protein import; required for Ub-dependent degradation of short-lived proteins; associated with the chaperonin-containing T-complex; unique specificity in propagation of [URE3] prions and vacuolar degradation of gluconeogenic enzymes; contributes to tRNA nuclear import during starvation; targeted to vacuoles via the AP-3 pathway; localizes to exosomes during heat stress with a role in thermotolerance; 98% identity with Ssa1p 2 3 4 5 6 7 8 9 10 11
- Name Description
- Stress-Seventy subfamily A 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ssa2p is 639 amino acids long, very high in abundance and slightly longer-lived; monomethylated on K421, monoacetylated on 21 residues, sumoylated on 4 residues, ubiquitinylated on 7 residues, succinylated on 26 residues, and phosphorylated on 36 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SSA2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the chaperonin-containing T-complex (CCT particle, TriC) that is predicted to be an ATPase; involved in protein folding and tRNA import into nucleus under starvation conditions
View computational annotations
Molecular Function
- Manually Curated
- enables ATP binding (IDA)
- enables ATP hydrolysis activity (ISS)
- enables tRNA binding (IDA)
Biological Process
- Manually Curated
- involved in cellular response to starvation (IMP)
- involved in proteasome-mediated ubiquitin-dependent protein catabolic process (IGI)
- involved in protein folding (IDA, IMP)
- involved in SRP-dependent cotranslational protein targeting to membrane, translocation (IMP)
- involved in tRNA import into nucleus (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in cytosol (IDA)
- is active in extracellular vesicle (IDA)
- located in fungal-type cell wall (IDA)
- located in fungal-type vacuole membrane (IDA)
- located in mitochondrion (IDA)
- High-Throughput
- located in mitochondrion (HDA)
- located in plasma membrane (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant is defective in protein targeting to vacuoles and in maintenance of [URE3] and [PSI+] prions; homozygous diploids are sensitive to actinomycin D and show increased innate thermotolerance; overexpression in Sigma1278b causes increased invasive growth
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Ssa2p interacts physically with proteins involved in transcription by RNA polymerase II; SSA2 interacts genetically with genes involved in protein targeting
815 total interactions for 588 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-02-06
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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