TCB1 / YOR086C Overview
- Standard Name
- TCB1 1
- Systematic Name
- YOR086C
- SGD ID
- SGD:S000005612
- Feature Type
- ORF , Verified
- Description
- Lipid-binding tricalbin; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of the phosphatase Sac1p to its PM substrate PI4P; regulates PM phospholipid homeostasis; contains 3 calcium and lipid-binding domains; localizes to the cortical ER and mitochondria; localizes to the nucleus-vacuole junction during glucose restriction; C-termini of Tcb2p and Tcb3p interact 2 3 4 5 6
- Name Description
- Three Calcium and lipid Binding domains (TriCalBins) 1
- Paralog
- TCB2 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TCB1/YOR086C is located on the right arm of chromosome XV between oligosaccharyltransferase complex subunit OST3 and tRNA-Asn gene YNCO0018W; coding sequence is 3561 nucleotides long with 31 SNPs, 10 of which cause amino acid polymorphisms; TCB1 has a paralog, TCB2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Tcb1p is 1186 amino acids long, low in abundance and average half-life; phosphorylated on 15 residues and ubiquitinylated on 4 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TCB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Lipid binding protein involved in regulation of phosphatidylinositol dephosphorylation, ER-to-Golgi ceramide transport, and ER membrane organization; localizes to plasma membrane, endoplasmic reticulum, and mitochondria
View computational annotations
Molecular Function
- Manually Curated
- enables lipid binding (IDA)
Biological Process
- Manually Curated
- involved in endoplasmic reticulum membrane organization (IGI)
- acts upstream of or within ER to Golgi ceramide transport (IMP)
- involved in maintenance of protein localization in endoplasmic reticulum (IGI)
- acts upstream of or within phospholipid homeostasis (IGI)
- involved in regulation of phosphatidylinositol dephosphorylation (IGI)
Cellular Component
- Manually Curated
- located in cortical endoplasmic reticulum (IDA)
- is active in nucleus-vacuole junction (IDA)
- High-Throughput
- located in cell periphery (HDA)
- located in endoplasmic reticulum (HDA)
- located in mitochondrion (HDA)
- located in plasma membrane (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- TCB1/YOR086C is a non-essential gene in reference strain S288C; null mutants are viable and show decreased desiccation resistance, increased competitive fitness, and abnormal vacuolar morphology; the null mutant of paralog tcb2 is viable; the tcb1 tcb2 double mutant displays a phenotypic enhancement
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Tcb1p interacts physically with proteins involved in chromatin organization; TCB1 interacts genetically with genes involved in lipid metabolism
179 total interactions for 159 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- TCB1/YOR086C transcription is regulated by Msn2p in response to heat
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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