RPB2 / YOR151C Overview
- Standard Name
- RPB2 1 2
- Systematic Name
- YOR151C
- SGD ID
- SGD:S000005677
- Aliases
- B150 14 , RPB150 10 , RPO22 9 11 , SIT2 8 , SOH2 12 13
- Feature Type
- ORF , Verified
- EC Number
- 2.7.7.6
- Description
- RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit 3 4 5 6 7
- Name Description
- RNA Polymerase B 2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rpb2p is 1224 amino acids long, moderate in abundance and slightly shorter-lived; monoacetylated on 13 residues, phosphorylated on 23 residues, and ubiquitinylated on 11 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RPB2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the RNA polymerase II core complex that contributes to RNA polymerase II activity; involved in transcription by RNA polymerase II
View computational annotations
Molecular Function
- Manually Curated
- enables DNA binding (IDA)
- contributes to DNA-directed RNA polymerase activity (IDA)
- contributes to RNA-directed RNA polymerase activity (IDA)
- enables zinc ion binding (RCA)
- High-Throughput
- enables mRNA binding (HDA)
Biological Process
- Manually Curated
- involved in transcription by RNA polymerase II (IMP)
Cellular Component
- Manually Curated
- is active in proteasome storage granule (IDA)
- part of RNA polymerase II, core complex (IDA)
- High-Throughput
- located in cytoplasmic stress granule (HDA)
- located in mitochondrion (HDA)
- located in nucleus (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in reference strain S288C; conditional mutants display inositol auxotrophy, cold and heat sensitivity, decreased vegetative growth, decreased mRNA accumulation, decreased RNA polymerase II core enzyme assembly, decreased resistance to 6-azauracil and mycophenolic acid, and decreased toxin resistance; dominant negative mutants have increased colony sectoring and decreased vegetative growth; reduction of function mutants have heat sensitivity, increased osmotic stress resistance, decreased utilization of maltose, decreased resistance to protein synthesis inhibitor cycloheximide and antifungal drug ketoconazole, and increased resistance to DNA-damaging agents including camptothecin and doxorubicin; overexpression causes decreased vegetative growth and decreased resistance to rapamycin; in large-scale studies, displays decreased oxidative stress resistance, increased competitive fitness, increased toxin resistance, and is haploinsufficient
Classical Genetics
- conditional
- null
- reduction of function
- dominant negative
- null
- overexpression
- reduction of function
- repressible
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rpb2p interacts physically with proteins involved in transcription by RNA polymerase II; RPB2 interacts genetically with genes involved in transcription by RNA polymerase II
766 total interactions for 523 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-04-29
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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