NHP6A / YPR052C Overview
- Standard Name
- NHP6A 1
- Systematic Name
- YPR052C
- SGD ID
- SGD:S000006256
- Feature Type
- ORF , Verified
- Description
- High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress 3 4 5 6 7 8 9 10
- Name Description
- Non-Histone Protein 2
- Paralog
- NHP6B 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- NHP6A has a paralog, NHP6B, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Nhp6ap is 93 amino acids long with 1 polymorphic residue, it is present at moderate abundance, with a shorter half-life; Nhp6a protein abundance increases in response to DNA replication stress
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NHP6A alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA and nucleosome-binding protein involved in chromatin remodeling, assembly of the RNA polymerase II and III pre-initiation complexes, and maintenance of transcriptional fidelity; localizes in the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA binding, bending (IDA)
- enables MutSalpha complex binding (IDA)
- enables nucleosome binding (IDA)
Biological Process
- Manually Curated
- involved in chromatin organization (IDA)
- involved in chromatin remodeling (IGI)
- involved in maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III (IDA)
- involved in RNA polymerase II preinitiation complex assembly (IDA)
- involved in RNA polymerase III preinitiation complex assembly (IGI, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- NHP6A/YPR052C is a non-essential gene in reference strain S288C; null mutants are viable and show compensatory increases in NHP6B mRNA and Nhp6bp protein, with reduced endocytosis. Drug responses are mixed: they are more sensitive to bleomycin and DMSO (and alborixin in heterozygotes), yet more resistant to cycloheximide, fenpropimorph, fluconazole, tebuconazole, and to the toxicity of Aβ42; oxidative stress resistance to paraquat is increased. Competitive fitness is elevated in minimal and synthetic complete media, while heterozygous diploids are haploinsufficient in a turbidostat assay. Overexpression reduces growth rate and can arrest growth under galactose induction.
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- NHP6A and NHP6B encode paralogous High Mobility Group (HMG) B domain-containing transcription factors that are involved in the modulation of chromatin structure. The Nhp6A and Nhp6B proteins differ at their N-termini, but over the 90 amino acid core region are 96% similar. Nhp6p contains a single HMGB domain that binds DNA in the minor groove and a basic N-terminal extension that wraps around DNA to contact the major groove, enabling Nhp6p to bind DNA as a monomer in a sequence-nonspecific manner, and bend DNA sharply. Nhp6p affects a range of processes linked to appropriate regulation of transcription through maintenance of normal chromatin structure. Nhp6p functions in the formation of preinitiation complexes at RNA polymerase II promoters, and also promotes RNA polymerase III transcription, as well as facilitating the activity of chromatin modifying complexes. Nhp6p localization in the vicinity of transcription start sites parallels that of nucleosomes. Expression of NHP6 genes is regulated by the concentration of Nhp6 protein or RNA, as overexpression of Nhp6p results in a dramatic decrease in NHP6 expression, and is toxic to cells.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-03-30
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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