YKE2 / YLR200W Overview
- Standard Name
- YKE2 1
- Systematic Name
- YLR200W
- SGD ID
- SGD:S000004190
- Aliases
- GIM1 2 , PFD6 5
- Feature Type
- ORF , Verified
- Description
- Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation 2 3 4
- Name Description
- Yeast ortholog of mouse KE2 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Yke2p is 114 amino acids long, low in abundance and slightly longer-lived; phosphorylated on 4 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all YKE2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Tubulin-binding subunit of prefoldin complex involved in protein folding and tubulin complex assembly; also activates transcription elongation; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables tubulin binding (IDA)
Biological Process
- Manually Curated
- involved in positive regulation of transcription elongation by RNA polymerase II (IMP, IGI)
- involved in protein folding (IMP)
- involved in tubulin complex assembly (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- part of prefoldin complex (IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant displays increased cell size, abnormally elongated buds, abnormal random budding pattern, slow growth, and delay in mitotic onset; displays loss of respiratory growth; increased mitotic chromosome loss, decreased mitotic recombination, and decreased Ty1 transposition; displays defective endocytosis and increased protein aggregation during arsenite stress; displays decreased resistance to cytoskeleton-damaging agent benomyl, DNA-damaging agent hydroxyurea, arsenite, zinc, rapamycin, protein synthesis inhibitor cordycepin, ethanol, fatty acids, Congo Red, and Aβ42 toxicity; displays increased resistance to some ER stress agents and DNA-damaging agents
Classical Genetics
- null
- bud morphology: abnormal
- budding pattern: abnormal
- cell shape: abnormal
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- endocytosis: decreased
- fermentative growth: decreased rate
- heat sensitivity: increased
- mitotic recombination: decreased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: absent
- stress resistance: decreased
- stress resistance: increased
- transposable element transposition: decreased
- vegetative growth: decreased rate
- viable
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Yke2p interacts physically with proteins involved in transcription by RNA polymerase II; YKE2 interacts genetically with genes involved in mitosis
1505 total interactions for 747 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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