Target of rapamycin complex 1 (TORC1) integrates nutrient signals with cell growth. While its inactivation is known to trigger mitotic slippage via APC/C-Cdh1-dependent securin degradation and separase activation, the molecular basis of cohesion loss during nutrient stress has remained incompletely defined. In budding yeast, we show that TORC1 inactivation elicits a noncanonical, proteasome-dependent degradation of cohesin that is independent of securin and separase. Separase was itself destabilized upon TORC1 inactivation, yet Scc1 degradation persisted even in a separase-resistant mutant. Cohesin degradation proceeds when APC/C is impaired, indicating involvement of an atypical ubiquitin ligase. These results reveal a second, aberrant route to sister chromatid dissociation upon TORC1 inactivation, operating in parallel with the previously reported APC/C-Cdh1 pathway, via the unconventional degradation of mitotic key factors.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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