In endosymbiosis, unicellular organisms, mostly prokaryotes, live inside the cells of a host that may be uni- or multicellular. More than half insect and arthropod species host the obligate endosymbiont Wolbachia spp., an alpha-proteobacterium that, given time, may coevolve with its host and progress from parasitism to strict mutualism, where an obligate endosymbiont avoids rejection by providing advantages to the host such as essential nutrients. It was recently found that in the wild yeasts may host both facultative and obligate endosymbionts. To model host-symbiont mutualist interactions in yeast, we tested two different Saccharomyces cerevisiae riboflavin-synthesis pathway deletion mutants (ΔRIB1 and ΔRIB4), finding that these failed to grow in the absence of exogenous riboflavin (RF), i.e., they were RF-auxotrophic strains. In both mutants Wolbachia infection cured auxotrophy. In addition, Wolbachia infection led to a mild increase in survival to exposure to 1 M NaCl or to 10 mM hydrogen peroxide. The artificial yeast/endosymbiont model proposed here may become a useful tool to explore parasitic, facultative and mutualist associations.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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