RTT101 / YJL047C Overview
- Standard Name
- RTT101 1
- Systematic Name
- YJL047C
- SGD ID
- SGD:S000003583
- Aliases
- CUL8 2 , CULC 2
- Feature Type
- ORF , Verified
- Description
- Cullin subunit of Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; functions as DPC (DNA-protein crosslink) repair factor throughout cell cycle; Rtt101p-Mms22p ligase associates with replisome complex during S phase via Ctf4p; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by ubiquitin-like protein Rub1p 2 3 4 5 6 7 8
- Name Description
- Regulator of Ty1 Transposition 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RTT101 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-protein transferase of the Cul8-RING ubiquitin ligase complex; involved in protein catabolism, rRNA decay, replication fork processing, RNA-mediated transposition, and regulation of mitotic nuclear division; localizes to the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin-protein transferase activity (IDA)
Biological Process
- Manually Curated
- involved in nonfunctional rRNA decay (IMP)
- involved in regulation of mitotic nuclear division (IMP)
- involved in replication fork processing (IMP)
- involved in transposable element silencing (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- RTT101/YJL047C is a non-essential gene in reference strain S288C; null mutants are viable but grow slowly in exponential phase with decreased utilization of carbon and nitrogen sources and decreased competitive fitness. Null mutants exhibit increased budding index, delayed cell cycle progression in mid-anaphase and through the G2/M phase transition, decreased G1 phase duration, variable protein/peptide modification (both increased and decreased), and increased protein/peptide accumulation. Morphological abnormalities include abnormal endoplasmic reticulum morphology, abnormal nuclear morphology, abnormal lipid particle morphology, and abnormal vacuolar morphology. Stress resistance phenotypes are context-dependent: null mutants show increased heat sensitivity but increased innate thermotolerance, decreased oxidative stress resistance, decreased desiccation resistance, decreased toxin resistance, decreased UV resistance, variable resistance to chemicals (both increased and decreased depending on the compound), and increased respiratory growth rate. Null mutants display decreased silencing, increased mitotic recombination, increased mutation frequency, increased or normal transposable element transposition (context-dependent), and decreased replicative lifespan. Overexpression of RTT101 results in decreased vegetative growth, decreased competitive fitness, increased invasive growth, decreased metal resistance, decreased UV resistance, and variable resistance to chemicals (both increased and decreased depending on the compound).
Classical Genetics
- null
- budding index: increased
- cell cycle progression in mid anaphase: delayed
- cell cycle progression through the G2/M phase transition: delayed
- growth in exponential phase: decreased rate
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- transposable element transposition: increased
- transposable element transposition: normal
- viable
- null
- budding index: increased
- cell cycle progression in G1 phase: decreased duration
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- haploproficient
- heat sensitivity: increased
- innate thermotolerance: increased
- lipid particle morphology: abnormal
- mitotic recombination: increased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: increased rate
- silencing: decreased
- toxin resistance: decreased
- transposable element transposition: increased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
471 total interactions for 295 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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