RTT101 / YJL047C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

RTT101/YJL047C is a non-essential gene in reference strain S288C; null mutants are viable but grow slowly in exponential phase with decreased utilization of carbon and nitrogen sources and decreased competitive fitness. Null mutants exhibit increased budding index, delayed cell cycle progression in mid-anaphase and through the G2/M phase transition, decreased G1 phase duration, variable protein/peptide modification (both increased and decreased), and increased protein/peptide accumulation. Morphological abnormalities include abnormal endoplasmic reticulum morphology, abnormal nuclear morphology, abnormal lipid particle morphology, and abnormal vacuolar morphology. Stress resistance phenotypes are context-dependent: null mutants show increased heat sensitivity but increased innate thermotolerance, decreased oxidative stress resistance, decreased desiccation resistance, decreased toxin resistance, decreased UV resistance, variable resistance to chemicals (both increased and decreased depending on the compound), and increased respiratory growth rate. Null mutants display decreased silencing, increased mitotic recombination, increased mutation frequency, increased or normal transposable element transposition (context-dependent), and decreased replicative lifespan. Overexpression of RTT101 results in decreased vegetative growth, decreased competitive fitness, increased invasive growth, decreased metal resistance, decreased UV resistance, and variable resistance to chemicals (both increased and decreased depending on the compound).

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources