YPC1 / YBR183W Overview
- Standard Name
- YPC1 1
- Systematic Name
- YBR183W
- SGD ID
- SGD:S000000387
- Feature Type
- ORF , Verified
- Description
- Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication 1 2 3
- Name Description
- Yeast Phyto-Ceramidase 1
- Paralog
- YDC1 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- YPC1 has a paralog, YDC1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all YPC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Bifunctional phytoceramidase/sphingosine N-acetyltranferase involved in ceramide metabolism; localizes to cortical ER
View computational annotations
Molecular Function
- Manually Curated
- enables N-acylsphingosine amidohydrolase activity (IMP, RCA)
- enables sphingosine N-acyltransferase activity (IGI, IMP)
Biological Process
- Manually Curated
- involved in ceramide biosynthetic process (IGI)
- involved in ceramide catabolic process (IMP)
- involved in sphingolipid biosynthetic process (RCA)
Cellular Component
- Manually Curated
- located in cortical endoplasmic reticulum (IDA)
- is active in cytosol (RCA)
- located in endoplasmic reticulum (IDA)
- High-Throughput
- located in endoplasmic reticulum (HDA)
Metabolic Pathways
Metabolic Pathways annotations describe biochemical pathways involving this gene product in small molecule metabolism. These annotations connect gene products to specific metabolic reactions, substrates, and products within larger metabolic networks. Metabolic pathway data is curated from published scientific literature. Click the links to see detailed pathway diagrams on SGD's YeastPathways site for further exploration.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- YPC1/YBR183W is a non-essential gene in reference strain S288C; null mutants are viable with normal competitive fitness in some conditions and haploproficiency in others, decreased vegetative growth, auxotrophy for certain nutrients, and decreased heat sensitivity. Null mutants exhibit abnormal and increased accumulation of certain chemical compounds, abnormal protein/peptide distribution, abnormal vacuolar morphology, decreased oxidative stress resistance, and decreased resistance to chemicals. Overexpression of YPC1 results in decreased and increased accumulation of different chemical compounds, abnormal mitochondrial morphology, decreased respiratory metabolism rate, decreased chronological lifespan, increased resistance to some chemicals but decreased resistance to others, decreased metal resistance, and decreased UV resistance.
Classical Genetics
- null
- overexpression
- null
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: normal
- haploproficient
- heat sensitivity: decreased
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast YPC1 is homologous to human ACER3 and has been used to study early childhood progressive leukodystrophy
Manually Curated
- leukodystrophy (IGI, ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-02-24
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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