BEM1 / YBR200W Overview
- Standard Name
- BEM1 1
- Systematic Name
- YBR200W
- SGD ID
- SGD:S000000404
- Aliases
- SRO1 8
- Feature Type
- ORF , Verified
- Description
- SH3 domain-containing scaffold protein; regulates the guanine exchange factor (GEF) activity of Cdc24p and Cla4p-mediated phosphorylation of Cdc24p, contributing to the regulation of Cdc42p, polarity axis establishment and morphogenesis; scaffold protein that utilizes multivalent lipid binding sites to target and concentrate complexes of polarity establishment proteins at sites of polarized growth during growth and mating; regulates homotypic vacuole fusion 2 3 4 5 6 7
- Name Description
- Bud EMergence 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- BEM1/YBR200W is located on the right arm of chromosome II, coding sequence is 1656 nucleotides long with 2 nonsynonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein containing SH3-domains; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BEM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Scaffold protein involved in establishing shmoo orientation and polarized cell growth; binds phosphatidylinositol-3-phosphate, phosphatidylinositol-4-phosphate and other phospholipids; directly activates the guanyl nucleotide exchange factor of Cdc24p and the protein kinase activity of Cla4p; positive regulator of homotypic vacuole fusion; localizes to incipient bud sites and sites of polarized growth such as the bud neck and bud tip
View computational annotations
Molecular Function
- Manually Curated
- enables guanyl nucleotide exchange factor activator activity (IDA)
- enables molecular adaptor activity (IPI)
- enables phosphatidylinositol-3-phosphate binding (IDA)
- enables phosphatidylinositol-4-phosphate binding (IDA)
- enables phospholipid binding (IDA)
- enables protein kinase activator activity (IDA)
Biological Process
- Manually Curated
- involved in cell morphogenesis involved in conjugation with cellular fusion (IMP)
- involved in establishment of cell polarity (IGI)
- involved in maintenance of protein location (IMP)
- involved in positive regulation of vacuole fusion, non-autophagic (IMP)
Cellular Component
- Manually Curated
- located in cell cortex (IDA)
- located in cellular bud neck (IDA)
- located in cellular bud tip (IDA)
- located in cytoplasm (IDA)
- located in incipient cellular bud site (IDA)
- located in site of polarized growth (IDA)
- High-Throughput
- located in mating projection tip (HDA)
- located in mitochondrion (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- BEM1/YBR200W is a non-essential gene in reference strain S288C, essential gene in strain Sigma1278b; null mutant grows slowly, fails to form shmoo under alpha factor treatment, shows reduced mating efficiency, decreased invasive growth and is unable to form pseudohyphae
Classical Genetics
- null
- axial budding pattern: decreased
- cell shape: abnormal
- chemical compound accumulation: decreased
- invasive growth: decreased
- mating efficiency: decreased
- prion formation: decreased
- protein/peptide distribution: decreased
- protein/peptide modification: decreased
- pseudohyphal growth: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- conditional
- unspecified
- reduction of function
- null
- alkaline pH resistance: decreased
- budding index: decreased
- cell size: increased
- chemical compound accumulation: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- inviable
- liquid culture appearance: abnormal
- metal resistance: decreased
- mitotic recombination: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- resistance to enzymatic treatment: increased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased
- viable
- unspecified
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
994 total interactions for 539 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-10-20
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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