RML2 / YEL050C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

RML2/YEL050C is a non-essential gene in reference strain S288C; null mutants are viable but grow slowly, form petites, and display haploinsufficiency, decreased cell size, and decreased competitive fitness. Null mutants exhibit abnormal chromosome/plasmid maintenance, absent or decreased mitochondrial genome maintenance, abnormal mitochondrial morphology, abnormal vacuolar morphology, absent or decreased respiratory growth, and decreased biofilm formation. Null mutants show abnormal, decreased, and increased accumulation of different chemical compounds and decreased utilization of nitrogen sources. Stress resistance phenotypes are context-dependent: null mutants display increased heat sensitivity, decreased innate thermotolerance, decreased metal resistance, increased oxidative stress resistance, variable overall stress resistance (increased in some conditions), variable resistance to chemicals (both increased and decreased depending on the compound), and variable toxin resistance (both increased and decreased depending on the toxin). Lifespan effects include decreased chronological lifespan but increased replicative lifespan. Conditional alleles show decreased respiratory growth and absent utilization of certain carbon sources. Overexpression of RML2 results in abnormal cell cycle progression.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources