SLT2 / YHR030C Overview
- Standard Name
- SLT2 1
- Systematic Name
- YHR030C
- SGD ID
- SGD:S000001072
- Aliases
- BYC2 , MPK1 12 , SLK2 , LYT2 13
- Feature Type
- ORF , Verified
- EC Number
- 2.7.11.24
- Description
- Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway 2 3 4 5 6 7 8 9 10 11
- Name Description
- Suppressor of the LyTic phenotype 1
- Paralog
- KDX1 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SLT2/YHR030C is located on the right arm of chromosome near the centromere between ARS809 replication origin and RRM3 DNA helicase involved in rDNA replication; coding sequence is 1455 nucleotides long with 10 SNPs and a variable 3' end
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Slt2p is 484 amino acids long, low in abundance and slightly longer-lived; dephosphorylated on T190, ubiquitinylated on K155 and K263, and phosphorylated on 13 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SLT2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- MAP kinase involved in control of cell wall integrity, regulation of proteasome assembly, response to osmotic stress and ER unfolded proteins; component of a MAP kinase regulatory cascade; localized to the cytoplasm and sites of cell wall growth (bud tips, bud necks, mating projections)
View computational annotations
Molecular Function
- Manually Curated
- enables protein serine/threonine kinase activity (IDA)
- High-Throughput
- enables protein kinase activity (HDA)
Biological Process
- Manually Curated
- involved in cell integrity MAPK cascade (IGI)
- involved in double-strand break repair via break-induced replication (IMP)
- involved in endoplasmic reticulum unfolded protein response (IDA, IMP)
- involved in fungal-type cell wall biogenesis (IGI)
- involved in IRE1-mediated unfolded protein response (IMP)
- involved in pexophagy (IMP)
- involved in positive regulation of division septum assembly (IGI)
- involved in regulation of cell size (IMP)
- involved in regulation of flocculation (IMP)
- involved in regulation of fungal-type cell wall organization (IMP, IGI)
- involved in regulation of proteasome assembly (IMP)
- involved in regulation of protein import into nucleus (IMP)
- involved in response to acidic pH (IMP)
- involved in response to endoplasmic reticulum stress (IMP)
- involved in signal transduction (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in cellular bud tip (IDA)
- located in mating projection tip (IDA)
- located in mitochondrion (IDA)
- located in nucleus (IDA)
- High-Throughput
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- SLT2/YHR030C is a non-essential gene; null mutants are viable but exhibit a wide range of phenotypic changes including decreased acid and alkaline pH resistance, abnormal cell wall morphology, decreased chronological lifespan, increased cold and heat sensitivity, decreased hydrostatic pressure resistance, decreased killer toxin and metal resistance, decreased mitophagy, decreased osmotic and oxidative stress resistance, absent pexophagy, decreased resistance to enzymatic treatment, abnormal RNA accumulation, increased toxin resistance, decreased biofilm formation, decreased cell size, decreased chitin deposition, decreased competitive fitness, decreased desiccation resistance, decreased endocytosis, decreased mating response, decreased prion formation, decreased respiratory growth, decreased stress resistance, decreased utilization of nitrogen sources, abnormal vacuolar morphology, and decreased rate of vegetative growth. Reduction of function mutants exhibit abnormal cell wall morphology, increased heat sensitivity, and decreased metal resistance. Conditional mutants show increased heat sensitivity, decreased hyperosmotic stress resistance, absent respiratory growth, and decreased toxin resistance. Repressible mutants display decreased toxin resistance. The slt2 null mutant is viable; the null mutant of paralog kdx1 is viable; the slt2 kdx1 double mutant displays a phenotypic enhancement.
Classical Genetics
- null
- acid pH resistance: decreased
- autophagy: normal
- auxotrophy
- cell cycle progression in G1 phase: delayed
- cell death: absent
- cell wall morphology: abnormal
- chemical compound accumulation: increased
- chronological lifespan: decreased
- cold sensitivity: increased
- desiccation resistance: increased
- heat sensitivity: increased
- heat sensitivity: normal
- hydrostatic pressure resistance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- mitophagy: decreased
- osmotic stress resistance: decreased
- oxidative stress resistance: decreased
- pexophagy: absent
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- resistance to chemicals: absent
- resistance to chemicals: decreased
- resistance to chemicals: normal
- resistance to enzymatic treatment: decreased
- RNA accumulation: abnormal
- RNA accumulation: decreased
- RNA accumulation: increased
- toxin resistance: increased
- reduction of function
- conditional
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- biofilm formation: decreased
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chitin deposition: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- mating response: decreased
- metal resistance: decreased
- prion formation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory growth: decreased rate
- stress resistance: decreased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- repressible
- conditional
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Slt2p interacts physically with proteins involved in transcription by RNA polymerase II; SLT2 interacts genetically with genes involved in transcription by RNA polymerase II
2064 total interactions for 1050 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-04-05
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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