STB5 / YHR178W Overview
- Standard Name
- STB5 1
- Systematic Name
- YHR178W
- SGD ID
- SGD:S000001221
- Feature Type
- ORF , Verified
- Description
- Transcription factor; regulates multidrug resistance and the oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro; negative regulator of autophagy, modulating the transcription of genes involved in NADPH production and the pentose phosphate pathway; Stb5p expression is reduced during nitrogen starvation and autophagy induction, while STB5 mRNA levels are significantly increased 1 2 3 4
- Name Description
- Sin Three Binding protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all STB5 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-binding transcription factor involved in regulation of transcription by RNA polymerase II. And transcription elongation by RNA polymerase II promoter; localizes to nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor activity (IDA, IMP)
- enables zinc ion binding (RCA)
- High-Throughput
- enables sequence-specific DNA binding (HDA)
Biological Process
- Manually Curated
- involved in cellular response to oxidative stress (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in transcription elongation by RNA polymerase II (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- STB5/YHR178W is a non-essential gene in reference strain S288C; deletion (stb5Δ) is viable but causes broadly reduced vegetative and competitive growth, cold sensitivity, abnormal vacuolar morphology in high salt, and impaired nitrogen metabolism (slower ammonium use, absent allantoin use) with myo‑inositol auxotrophy. Metabolic/redox balance shifts: NADPH decreases in stb5Δ (increases with overexpression), zinc accumulates while zinc tolerance falls, RNA/protein homeostasis is altered, and autophagy rises under nitrogen starvation; transposon activity is also perturbed. Stress phenotypes are mixed: oxidative stress tolerance generally drops (paraquat, H2O2, azobis DMF), yet thermotolerance improves (reduced heat sensitivity and enhanced survival after heat shock), and chronological lifespan increases. stb5Δ is hypersensitive to many chemicals including caffeine, SDS, cycloheximide/hygromycin B, arsenite, chromium, acetaldehyde, glyoxal, acrolein, pentanol, 5‑fluorouracil, cordycepin, sorbate, DMSO, and zinc, but shows increased resistance to sirolimus/rapamycin, myriocin, 2,4‑diacetylphloroglucinol, and certain metals (cadmium, mercury). Toxin responses vary, with decreased resistance to Aβ42.
Classical Genetics
- null
- autophagy: increased
- budding index: decreased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- cold sensitivity: increased
- hyperosmotic stress resistance: decreased
- metal resistance: increased
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: decreased
- RNA accumulation: increased
- starvation resistance: decreased
- utilization of nitrogen source: decreased
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
- null
- auxotrophy
- chronological lifespan: increased
- competitive fitness: decreased
- heat sensitivity: decreased
- innate thermotolerance: increased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- starvation resistance: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- transposable element transposition: increased
- utilization of nitrogen source: absent
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- repressible
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
919 total interactions for 683 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- STB5 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Stb5p activates transcription of genes involved in the pentose phosphate pathway and the regulation of NADPH production. It is required for normal resistance to acetaldehyde, diamide, and other oxidizing agents. It is also responsive to stress, binding to a wider range of genomic sites under oxidative stress conditions than under normal conditions. Most of the known targets of Stb5p, such as genes of the pentose phosphate and ergosterol biosynthetic pathways and the ATR1 and PDR12 drug efflux pumps, are transcriptionally activated by Stb5p. However, Stb5p represses transcription of at least one gene, PGI1. As a heterodimer with Pdr1p, Stb5p also activates transcription of genes involved in pleiotropic drug resistance, such as the ATP-binding cassette transporter genes PDR5, SNQ2, and YOR1, whose products mediate drug efflux from the cell. Stb5p binds to its own promoter, suggesting that its transcription is autoregulated.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
Loading literature…