CST6 / YIL036W Overview
- Standard Name
- CST6 1
- Systematic Name
- YIL036W
- SGD ID
- SGD:S000001298
- Aliases
- SHF1 8 , ACA2 3
- Feature Type
- ORF , Verified
- Description
- Basic leucine zipper (bZIP) transcription factor from ATF/CREB family involved in stress-responsive regulatory network; mediates transcriptional activation of NCE103 in response to low CO2 levels; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication 1 2 3 4 5 6 7
- Name Description
- Chromosome STability 1
- Paralog
- ACA1 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CST6/YIL036W is located on the left arm of chromosome IX between PRM2 pheromone-regulated protein and CKA1 alpha catalytic subunit of casein kinase 2; coding sequence is 1764 nucleotides long with one in-frame trinucleotide insertion in strains SK1 and Y55, and 25 SNPs, 12 of which cause amino acid polymorphisms; CST6 has paralog ACA1 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cst6p is 587 amino acids long, short-lived, low in abundance; contains disordered regions at both termini; sumoylated on K403 and K547, ubiquitinylated on K178 and K349, phosphorylated on 24 residues; stains SK1 and Y55 have an inserted alanine residue after Ala47; relocalizes to the cytosol in response to hypoxia
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CST6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding RNA polymerase II transcription factor involved in the cellular responses to carbon dioxide and oleic acid
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to carbon dioxide (IMP)
- involved in cellular response to oleic acid (IMP)
- involved in cellular response to stress (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IDA, IMP)
- involved in regulation of transcription by RNA polymerase II (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- CST6/YIL036W is a non-essential gene; null mutant is viable, slow-growing, cold-sensitive, has reduced competitive fitness, and increased sensitivity to oxidative stress and ethanol, increased resistance to benomyl; overexpression results in elevated mitotic recombination
Classical Genetics
- overexpression
- null
- unspecified
- null
- auxotrophy
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- heat sensitivity: increased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: decreased
- telomere length: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CST6 encodes a transcriptional activator of the ATF/CREB family, containing a bZIP structural motif which consists of a leucine zipper that mediates dimerization and an adjacent basic region that contacts DNA. Cst6p binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA, and with comparable efficiency to ATF/CREB sites with single or double substitutions at positions +/- 2. Cst6p can also bind ATF/CREB sites as a heterodimer with Aca1p. Cst6p is important for a variety of biological processes including growth on nonoptimal carbon sources and resistance to a variety of drugs. Target genes include GRE2 and COS8. Cst6p-dependent activation is regulated to some extent by carbon source.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-03-19
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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