MED2 / YDL005C Overview
- Standard Name
- MED2 1
- Systematic Name
- YDL005C
- SGD ID
- SGD:S000002163
- Feature Type
- ORF , Verified
- Description
- Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia 1 2 3
- Name Description
- MEDiator complex 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- MED2/YDL005C is located on the left arm of chromosome IV; coding sequence is 1296 nucleotides long with 10 nonsynonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes to the cytosol in response to hypoxia
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MED2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the core mediator complex, which acts as a bridge between transcription factors and RNA Pol II; binds RNA Pol II-specific transcription factors and has transcription coactivator activity; involved in assembly of the RNA Pol II preinitiation complex, regulation of Pol II transcription, response to ER stress, and replicative senescence
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in negative regulation of transcription by RNA polymerase II (IPI)
- involved in positive regulation of transcription by RNA polymerase II (IDA)
- acts upstream of or within response to endoplasmic reticulum stress (IMP)
- involved in RNA polymerase II preinitiation complex assembly (IDA)
Cellular Component
- Manually Curated
- part of core mediator complex (IDA)
- located in cytosol (IDA)
- part of mediator complex (IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- MED2/YDL005C is a non-essential gene in reference strain S288C; null mutants are viable but grow slowly and display decreased respiratory growth, decreased endocytosis, abnormal lipid particle morphology, and abnormal vacuolar morphology. Stress resistance phenotypes show increased heat sensitivity, decreased acid pH resistance, decreased alkaline pH resistance, decreased osmotic and hyperosmotic stress resistance, decreased oxidative stress resistance, decreased starvation resistance, and decreased killer toxin resistance. Null mutants also exhibit decreased replicative lifespan, decreased utilization of sulfur sources, and decreased transposable element transposition. Overexpression of MED2 results in abnormal silencing and increased replicative lifespan.
Classical Genetics
- null
- chemical compound accumulation: increased
- killer toxin resistance: decreased
- metal resistance: decreased
- metal resistance: increased
- osmotic stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- RNA accumulation: increased
- RNA modification: increased
- utilization of sulfur source: decreased
- overexpression
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- chemical compound accumulation: abnormal
- endocytosis: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: decreased
- lipid particle morphology: abnormal
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased
- starvation resistance: decreased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
272 total interactions for 155 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MED2 encodes a component of the tail module of the Mediator transcriptional regulation complex. The Mediator complex is highly conserved from yeast to human, and is a central player in the transcriptional regulation of protein-coding genes, being required for nearly all transcription by RNA polymerase II (RNAPII). Mediator bridges activators and the general transcription machinery. The core Mediator complex contains three distinct head, middle, and tail modules. Med2p, Nut1p, Pgd1p, Gal11p, and Sin4p are part of the tail module, which has a gene-selective, as opposed to a general, role in transcription, and is largely dedicated to the activation of genes involved in cellular responses to environmental signals. Med2p, Pgd1p, and Gal11p interact with gene-specific transcriptional activators such as Gcn4p. By interacting with activators, Mediator tail module subunits signal through the head and middle modules, which interact with general transcription factors and RNAPII subunits, to the basal transcription machinery to regulate transcription initiation. The tail module preferentially targets stress-regulated, TATA-containing, SAGA- and Swi/Snf-dependent genes, and may function along with specific chromatin regulators to provide a platform for preinitiation complex (PIC) formation at these highly regulated loci. Subunits of the Mediator tail module are also required for the activation of metabolic genes.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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