KCS1 / YDR017C Overview
- Standard Name
- KCS1 1
- Systematic Name
- YDR017C
- SGD ID
- SGD:S000002424
- Feature Type
- ORF , Verified
- EC Number
- 2.7.4.21
- Description
- Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, RNA polymerase I-mediated rRNA transcription and telomere maintenance; phosphorylated by the AMPK Snf1p, leading to reduced levels of pyrophosphorylated IP7, increased filamentous and invasive growth and increased cell elongation 2 3 4 5 6 7
- Name Description
- pKC1 Suppressor 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- KCS1/YDR017C is located on the right arm of chromosome IV; coding sequence is 3153 nucleotides long with 12 nonsynonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Kcs1p interacts physically with proteins involved in mitotic cell cycle; KCS1 interacts genetically with genes involved in transcription
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all KCS1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic inositol hexakisphosphate and heptakisphosphate kinase involved in biosynthesis of inositol phosphate
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in inositol phosphate biosynthetic process (IDA, IMP, RCA)
- acts upstream of or within intracellular phosphate ion homeostasis (IMP)
- involved in regulation of inositol phosphate biosynthetic process (IMP)
Metabolic Pathways
Metabolic Pathways annotations describe biochemical pathways involving this gene product in small molecule metabolism. These annotations connect gene products to specific metabolic reactions, substrates, and products within larger metabolic networks. Metabolic pathway data is curated from published scientific literature. Click the links to see detailed pathway diagrams on SGD's YeastPathways site for further exploration.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- KCS1/YDR017C is a non-essential gene; null mutants are viable but exhibit a variety of phenotypes including auxotrophy, increased cell size, abnormal chromosome/plasmid maintenance, increased cold sensitivity, increased heat sensitivity, decreased competitive fitness, abnormal endocytosis, decreased rate of fermentative growth, decreased growth rate in the exponential phase, decreased resistance to hyperosmotic stress, decreased innate thermotolerance, decreased invasive growth, decreased ionic stress resistance, decreased metal resistance, abnormal mitochondrial morphology, increased nutrient uptake, decreased pseudohyphal growth, absent or decreased sporulation, altered transposable element transposition, decreased utilization of carbon and nitrogen sources, abnormal vacuolar morphology, and decreased rate of vegetative growth. Conditional mutants also show increased heat sensitivity. Overexpression of KCS1 results in decreased vegetative growth rate and abnormal pseudohyphal growth.
Classical Genetics
- null
- cell size: increased
- chemical compound accumulation: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: delayed
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- endocytosis: abnormal
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- invasive growth: decreased
- ionic stress resistance: decreased
- metal resistance: decreased
- nutrient uptake: increased
- protein/peptide distribution: abnormal
- pseudohyphal growth: decreased
- resistance to chemicals: decreased
- RNA accumulation: increased
- small molecule transport: absent
- small molecule transport: decreased rate
- utilization of carbon source: decreased
- vacuolar morphology: abnormal
- conditional
- unspecified
- overexpression
- null
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- cold sensitivity: increased
- competitive fitness: decreased
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- mitochondrial morphology: abnormal
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation: absent
- sporulation: decreased
- transposable element transposition: decreased
- transposable element transposition: increased
- utilization of carbon source: decreased
- utilization of nitrogen source: absent
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
337 total interactions for 304 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-01-22
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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