BTN2 / YGR142W Overview
- Standard Name
- BTN2 1
- Systematic Name
- YGR142W
- SGD ID
- SGD:S000003374
- Feature Type
- ORF , Verified
- Description
- Compartment-specific sequestrase chaperone; small hsp that sequesters proteins at intranuclear quality control sites (INQs); sumoylated form promotes protein clearance from INQs during recovery from genotoxic stress; v-SNARE binding protein that mediates late endosome-Golgi protein sorting; modulates arginine uptake and pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; role in prion curing; expression after severe ethanol stress is involved in clearance of denatured proteins 2 3 4 5 6 7 8 9 10 11
- Name Description
- BaTteN disease 1
- Paralog
- CUR1 12
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- BTN2/YGR142W is located on the right arm of chromosome VII between vacuolar protein sorting protein VPS62 and YGRWdelta22; coding sequence is 1233 nucleotides long with 10 synonymous and 14 nonsynonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Btn2p is 410 amino acids long and low in abundance; phosphorylated on 7 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BTN2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- SNARE-binding protein involved in protein folding and transport; localizes to various cellular regions, including nucleus, late endosome, and cytosol
View computational annotations
Molecular Function
- Manually Curated
- enables protein sequestering activity (IMP)
- enables protein-folding chaperone binding (IPI)
- enables SNARE binding (IDA, IPI)
Biological Process
- Manually Curated
- involved in amino acid transport (IMP)
- involved in intracellular protein transport (IMP, IPI)
- involved in protein folding (IGI, IPI, IMP)
- involved in protein localization to nucleus (IMP, IPI)
- involved in regulation of pH (IMP)
- involved in retrograde transport, endosome to Golgi (IMP)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- located in late endosome (IDA)
- located in nuclear periphery (IDA)
- located in nucleus (IDA)
- located in perinuclear region of cytoplasm (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- BTN2/YGR142W is a non-essential gene in reference strain S288C; null mutant shows defects in protein sorting, reduced uptake of arginine, elevated formation of [URE3] prion, decreased sensitivity to Calcofluor White, and increased sensitivity to ethanol; overexpression causes abnormal budding and sensitivity to acidic conditions; the btn2 null mutant is viable; the null mutant of paralog cur1 is viable; the btn2 cur1 double mutant is viable
Classical Genetics
- null
- acid pH resistance: decreased
- adhesion: decreased
- biofilm formation: increased
- fermentative metabolism: abnormal
- hydrostatic pressure resistance: decreased
- nutrient uptake: increased
- prion formation: increased
- prion loss: decreased
- protein activity: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: increased
- viable
- overexpression
- reduction of function
- unspecified
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Btn2p interacts physically with proteins involved in Golgi vesicle transport; BTN2 interacts genetically with genes involved in protein folding
131 total interactions for 90 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- BTN2/YGR142W transcription is positively regulated by Hsf1p in response to heat, positively regulated by Znf1p during D-xylose fermentation, positively regulated by Ume6p during the meiotic cell cycle, and regulated by Ada2p, Fkh2p, Gcn5p, Med2p, Ncb2p, Nhp6ap, Reb1p, Rgr1p, Spt3p, Spt6p, Spt7p, Srb5p, Sua7p, Ume6p, Xbp1p, and Yap1p in response to heat
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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