MPS3 / YJL019W Overview
- Standard Name
- MPS3 1
- Systematic Name
- YJL019W
- SGD ID
- SGD:S000003556
- Aliases
- NEP98 2 , YJL018W
- Feature Type
- ORF , Verified
- Description
- Nuclear envelope protein; required for SPB insertion, SPB duplication, Kar5p localization near the SPB and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology) 1 2 3 4 5 6 7 8
- Name Description
- MonoPolar Spindle 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- MPS3/YJL019W is located on the left arm of chromosome X between dioxygenase TPH3 and myosin-like protein BBC1; coding sequence is 2049 nucleotides long with insertions and deletions, depending on strain, at nucleotides 1177..1189 as well as 13 SNPs, 4 of which cause amino acid polymorphisms; merged ORF with NEP98/YJL018W due to a single G nucleotide insertion in some strains
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mps3p is 682 amino acids long, low in abundance and slightly shorter-lived; monoacetylated on K557, N-glycosylated on N278, and phosphorylated on 9 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MPS3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in spindle pole body function, nuclear envelope organization and nuclear migration; participates in tethering of telomeres to nuclear envelope during meiosis; integral to nuclear envelope, localized to spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in establishment of mitotic sister chromatid cohesion (IMP)
- involved in homologous chromosome pairing at meiosis (IMP, IGI)
- involved in karyogamy (IMP)
- involved in meiotic telomere clustering (IGI, IMP)
- involved in mitotic sister chromatid cohesion (IMP)
- involved in nuclear migration involved in conjugation with cellular fusion (IMP)
- involved in spindle pole body duplication (IMP)
- involved in subtelomeric heterochromatin formation (IMP)
- involved in telomere tethering at nuclear periphery (IMP)
Cellular Component
- Manually Curated
- located in chromosome, telomeric region (IDA)
- located in half bridge of spindle pole body (IDA)
- located in membrane (IDA)
- located in nuclear envelope (IDA)
- located in nuclear periphery (IDA)
- located in spindle pole body (IDA)
- High-Throughput
- located in nuclear periphery (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in reference strain S288C; conditional mutants arrest in mitosis with monopolar spindles due to spindle pole body duplication defects, with most SUN domain mutants showing 80-90% large-budded cells at restrictive temperature; conditional mutants display defective nuclear fusion during mating with only 60-70% of zygotes completing karyogamy compared to 95% in wild-type; conditional mutants spontaneously diploidize at permissive temperature; meiotic defects include delayed onset, reduced telomere clustering, and abnormal chromosome pairing; conditional mutants show aberrant protein localization including reduced Cdc31p and Spc42p at spindle pole bodies and mislocalized telomeric proteins; conditional mutants are sensitive to benomyl and hydroxyurea; is haploinsufficient; overexpression decreases growth rate; in large-scale studies, displays increased nucleolar size and fragmentation
Classical Genetics
- repressible
- conditional
- reduction of function
- null
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mps3p interacts physically with proteins involved in transcription by RNA polymerase II; MPS3 interacts genetically with genes involved in chromatin organization
706 total interactions for 503 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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