RSC2 / YLR357W Overview
- Standard Name
- RSC2 1
- Systematic Name
- YLR357W
- SGD ID
- SGD:S000004349
- Feature Type
- ORF , Verified
- Description
- Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication 2 3 4 5 7
- Name Description
- Remodel the Structure of Chromatin 6
- Paralog
- RSC1 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- RSC2/YLR357W is located on the right arm of chromosome XII between ATG33 mitophagy-specific protein and ADE13 adenylosuccinate lyase; coding sequence is 2670 nucleotides long with 16 SNPs, 6 of which cause amino acid polymorphisms; RSC2 has a paralog, RSC1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rsc2p is 889 amino acids long, low in abundance, slightly longer-lived; contains disordered regions near both termini; acetylated on K651, sumoylated on 3 lysines, phosphorylated on 29 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RSC2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the RSC chromatin remodeling complex, slides along DNA affecting nucleosome positioning and transcription regulation; also involved in maintaining chromosome transmission fidelity as well as DNA repair via UV-damage excision, homologous recombination, and nonhomologous end joining
View computational annotations
Molecular Function
- Manually Curated
- enables chromatin binding (IDA)
Biological Process
- Manually Curated
- involved in chromosome segregation (IGI)
- involved in double-strand break repair via homologous recombination (IMP)
- involved in double-strand break repair via nonhomologous end joining (IPI)
- involved in nucleosome disassembly (IDA)
- involved in sister chromatid cohesion (IMP)
- involved in transcription elongation by RNA polymerase II (IDA)
- involved in UV-damage excision repair (IMP)
Cellular Component
- Manually Curated
- part of RSC-type complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- RSC2/YLR357W is a non-essential gene in reference strain S288C, null mutants are viable but show broad defects in growth, stress tolerance, and genome maintenance. Null and conditional alleles confer heat sensitivity (33–37°C), impaired respiration (absent growth on ethanol at 37°C and poor growth on glycerol), slower fermentative growth, and reduced competitive fitness across rich and synthetic media. Cells display abnormal chromosome/plasmid maintenance, shortened telomeres, increased transcriptional silencing, and altered cell biology, including enlarged cells and nucleoli, abnormal buds and vacuoles (exacerbated by high salt), increased RNA and glycogen accumulation, higher reactive oxygen species with SDS, elevated glutathione excretion, abnormal protein modification and distribution, and increased protein secretion. Deletion strains are myo‑inositol auxotrophs, have decreased chronological lifespan, and altered colony appearance; they also use galactose and nitrogen sources (allantoin, proline) more slowly. rsc2Δ is broadly hypersensitive to many chemicals and stresses (DNA damage, translation/transcription inhibitors, cell wall/membrane stressors, solvents, and several metals), yet shows increased resistance to select agents (e.g., tunicamycin, camptothecin, quinine, palmitoleic acid, mycophenolic acid, 2,4‑diacetylphloroglucinol, nickel sulfate, yttrium chloride), with cycloheximide resistance varying by assay. Killer toxin resistance is reduced, but resistance to in vivo EcoRI expression is increased. Overexpression decreases growth and fitness and enhances invasive growth in Sigma1278b; missense alleles D461G and V457M also cause heat sensitivity.
Classical Genetics
- null
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- respiratory growth: decreased
- RNA accumulation: increased
- silencing: increased
- UV resistance: decreased
- conditional
- null
- auxotrophy
- bud morphology: abnormal
- cell size: increased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- fermentative growth: decreased rate
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- protein secretion: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- respiratory growth: absent
- size of nucleolus: increased
- stress resistance: increased
- telomere length: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- utilization of sulfur source: absent
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rsc2p interacts physically with proteins involved in chromatin organization, RSC2 interacts genetically with genes involved in transcription; the rsc2 null mutant is viable, and the null mutant of paralog rsc1 is viable, as is the rsc2 rsc1 double mutant.
573 total interactions for 308 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2026-08-10
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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