PKR1 / YMR123W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

PKR1/YMR123W is a non-essential gene in reference strain S288C; null mutants are viable but display decreased vegetative growth rate, decreased utilization of carbon, iron, and nitrogen sources, auxotrophy for certain nutrients, and variable respiratory growth (absent, decreased, or increased depending on conditions). Null mutants exhibit abnormal, decreased, and increased accumulation of different chemical compounds, increased chemical compound excretion, increased chitin deposition, and abnormal vacuolar morphology. Stress resistance phenotypes are context-dependent: null mutants show decreased resistance to metals, chemicals, and toxins in most conditions, though resistance to some chemicals is increased or normal, decreased alkaline pH resistance, and decreased starvation resistance, while overall stress resistance is increased in some contexts. Null mutants display decreased chronological lifespan, decreased competitive fitness in most conditions (though exhibiting haploproficiency in some), and decreased transposable element transposition. Overexpression of PKR1 results in increased filamentous growth.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources