MSS11 / YMR164C Overview
- Standard Name
- MSS11 1
- Systematic Name
- YMR164C
- SGD ID
- SGD:S000004774
- Feature Type
- ORF , Verified
- Description
- Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1 1 2 3 4
- Name Description
- Multicopy Suppressor of STA genes 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mss11p is 758 amino acids long and extremely low in abundance; phosphorylated on 12 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MSS11 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in positive regulation of transcription by RNA polymerase II; localizes to nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in positive regulation of flocculation (IGI)
- involved in positive regulation of invasive growth in response to glucose limitation (IGI, IDA, IMP)
- involved in positive regulation of pseudohyphal growth (IMP)
- involved in positive regulation of starch catabolic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IGI, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant has severe growth defects under sulfur and nitrogen limitation and is unable to grow on nonfermentable carbon sources at elevated temperature; heat sensitive at 37°C; decreased resistance to cadmium, mercury, zinc, rapamycin, amitrole, myriocin, epoxiconazole, and doxorubicin but increased resistance to polygodial; decreased competitive fitness and decreased proline utilization; overexpression causes G1 arrest, is lethal, and can restore flocculation and invasive growth to S288C; in large-scale studies, displays increased metal reductase activity and defective vacuolar fragmentation; in Sigma1278b, null mutant completely lacks biofilm formation, flocculation, invasive growth, and pseudohyphal growth with decreased FLO11 mRNA levels and smoother colony morphology, while overexpression increases flocculation and invasive growth
Classical Genetics
- null
- overexpression
- null
- biofilm formation: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- heat sensitivity: increased
- invasive growth: absent
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- RNA accumulation: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mss11p interacts physically with proteins involved in transcription by RNA polymerase II; MSS11 interacts genetically with genes involved in transcription by RNA polymerase II
110 total interactions for 81 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MSS11 encodes a transcription factor that lacks overall similarity to known transcription regulator families, although it has similarity to Flo8p. Mss11p activates transcription of genes required for filamentous growth and adhesion. It is one of many regulators of FLO11, but seems to play a central role as it is absolutely required for FLO11 transcription. Other targets of Mss11p activation include the flocculin gene FLO1; the STA2 gene encoding a glucoamylase required for starch degradation; and cell wall mannoprotein genes such as TIR2, TIR3, and TIR4.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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