TRS130 / YMR218C Overview
- Standard Name
- TRS130 1
- Systematic Name
- YMR218C
- SGD ID
- SGD:S000004831
- Feature Type
- ORF , Verified
- Description
- Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic 1 2 3 4
- Name Description
- TRapp Subunit 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Trs130p is 1102 amino acids long, low in abundance and slightly shorter-lived; ubiquitinylated at K66, phosphorylated at S259 and S415
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TRS130 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the TRAPPII complex that contributes to Rab family guanyl-nucleotide exchange factor activity; involved in intra-Golgi and early endosome to Golgi vesicle-mediated transport; role in macroautophagy and the CVT pathway; localizes to the early endosome and the trans Golgi network
View computational annotations
Molecular Function
- Manually Curated
- contributes to guanyl-nucleotide exchange factor activity (IMP, IGI, IPI)
Biological Process
- Manually Curated
- involved in cytoplasm to vacuole targeting by the Cvt pathway (IMP)
- involved in early endosome to Golgi transport (IMP)
- involved in intra-Golgi vesicle-mediated transport (IMP)
- involved in macroautophagy (IMP)
Cellular Component
- Manually Curated
- located in early endosome (IDA)
- located in trans-Golgi network (IDA)
- part of TRAPPII protein complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in reference strain S288C; heat-sensitive mutants display absent autophagy and protein secretion, decreased protein transport including carboxypeptidase Y, decreased vacuolar transport, abnormal Golgi and endomembrane system morphology, abnormal protein distribution including enhanced Ypt1p punctate distribution and diffuse Ypt31p distribution, and chromosome instability; conditional mutants have decreased resistance to DNA-damaging agent camptothecin and increased resistance to antifungal drug micafungin; overexpression causes decreased resistance to radicicol and decreased UV resistance; in large-scale studies, displays decreased competitive fitness and is haploinsufficient
Classical Genetics
- conditional
- null
- reduction of function
- conditional
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Trs130p interacts physically with proteins involved in Golgi vesicle transport; TRS130 interacts genetically with genes involved in Golgi vesicle transport
211 total interactions for 127 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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