CRZ1 / YNL027W Overview
- Standard Name
- CRZ1 1
- Systematic Name
- YNL027W
- SGD ID
- SGD:S000004972
- Aliases
- HAL8 6 , TCN1 1 2
- Feature Type
- ORF , Verified
- Description
- Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium 1 2 3 4 5
- Name Description
- Calcineurin-Responsive Zinc finger 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CRZ1/YNL027W is located on the right arm of chromosome XIV between SAM complex component SAM50 and dubious ORF YNL028W; coding sequence is 2037 nucleotides long; gene structure includes an insertion in reference strain Y55 and several deletions in different alternative reference strains, with 12 synonymous, 5 nonsynonymous, and 2 untranslatable SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Crz1p is 678 amino acids long, low in abundance and slightly shorter-lived; ubiquitinylated on K71, sumoylated on K346 and K503, and phosphorylated on 47 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CRZ1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA-binding RNA polymerase II transcription activator involved in ion homeostasis, cell wall chitin biosynthesis, and the cellular response to blue light; involved in positive regulation of transcription in response to calcium ions, increased salt, alkaline pH, and pheromones; localizes to the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- High-Throughput
- enables sequence-specific DNA binding (HDA)
Biological Process
- Manually Curated
- involved in cellular hyperosmotic salinity response (IMP)
- involved in cellular response to alkaline pH (IMP)
- involved in cellular response to blue light (IDA)
- involved in cellular response to calcium ion (IGI, IMP)
- involved in cellular response to pheromone (IMP)
- involved in chitin biosynthetic process (IGI)
- involved in intracellular potassium ion homeostasis (IGI, IMP, IDA)
- involved in monoatomic ion homeostasis (IGI, IMP)
- involved in positive regulation of cellular response to drug (IMP)
- involved in positive regulation of sodium ion transport (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IGI, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant is sensitive to various ions including lithium, manganese, sodium, and calcium; in large-scale studies the null mutant shows sensitivity to various chemicals and shows either increased or decreased competitive fitness during growth on different media in different experiments
Classical Genetics
- null
- overexpression
- null
- alkaline pH resistance: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- silencing: decreased
- size of nucleolus: decreased
- stress resistance: decreased
- toxin resistance: decreased
- toxin resistance: increased
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Crz1p interacts physically with proteins involved in organelle assembly; CRZ1 interacts genetically with genes involved in transcription by RNA polymerase II
275 total interactions for 226 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CRZ1 encodes a zinc-coordinating transcription factor of the beta-beta-alpha zinc finger class that binds 5'-MWMMGCCMC-3' motifs. Crz1p regulates transcription of genes involved in cell wall biosynthesis, small-molecule transport, and the synthesis of membrane lipids and ergosterol in response to stress. Activity of Crz1p is positively controlled by the highly conserved Ca2+- and calmodulin-dependent protein phosphatase calcineurin. Crz1p is fully phosphorylated, localized to the cytosol, and transcriptionally inactive under non-stimulating conditions. Under various stresses, such as high temperature, high osmolarity, ethanol, alkaline pH and ER stress, Ca2+ influx is stimulated, followed by dephosphorylation of Crz1p at several residues, leading to a conformational change that hides a nuclear export signal and exposes a nuclear localization signal (recognized respectively by the beta-importins Msn5p and Nmd5p). After transport of dephosphorylated Crz1p into the nucleus, the Zn-finger domain binds specific DNA sequences (termed CDREs for Crz1p-dependent response elements) present within the promoter regions of target genes. Inducible targets of Crz1p include CRZ1 itself, RCN1 and RCN2 that encode positive and negative regulators of calcineurin, CMK2 that encodes a Ca2+/calmodulin-dependent protein kinase, PMC1, PMR1, and ENA1 that encode several P-type cation pumps responsible for efflux of Ca2+ , Mn2+ , Na+ , and Li+, and up to 100 additional genes involved in other processes.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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