RFC4 / YOL094C Overview
- Standard Name
- RFC4 1
- Systematic Name
- YOL094C
- SGD ID
- SGD:S000005454
- Feature Type
- ORF , Verified
- Description
- Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia 1 2 3
- Name Description
- Replication Factor C 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rfc4p is 323 amino acids long, low in abundance and long-lived; has AAA+ ATPase domain; relocalizes to the cytosol in response to hypoxia; sumoylated on K180, phosphorylated on S6, S156 and S263
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RFC4 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein subunit of the CTF18-RTF, DNA replication factor C, ELG1-RFC, and Rad17 RFC-like complexes that contributes to DNA clamp loading; involved in leading strand elongation and sister chromatid cohesion; localized to the nucleus; localized to cytosol during cellular response to hypoxia
View computational annotations
Molecular Function
- Manually Curated
- contributes to DNA clamp loader activity (IDA)
Biological Process
- Manually Curated
- involved in leading strand elongation (IDA)
- involved in sister chromatid cohesion (IDA)
Cellular Component
- Manually Curated
- part of Ctf18 RFC-like complex (IPI)
- located in cytosol (IDA)
- part of DNA replication factor C complex (IDA)
- part of Elg1 RFC-like complex (IPI)
- located in nucleus (IDA)
- part of Rad17 RFC-like complex (IDA, IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in reference strain S288C; heat-sensitive mutants display abnormal chromosome segregation, chromosome instability, increased mutation frequency, and decreased resistance to DNA-damaging agent camptothecin; cold-sensitive mutants have decreased resistance to DNA-damaging agents including methyl methanesulfonate and hydroxyurea and decreased UV resistance; rfc4-2 allele has abnormal S phase progression with reduced Rad53 phosphorylation and decreased resistance to DNA-damaging agents; overexpression causes decreased resistance to multiple stresses including acid pH, hyperosmotic stress, and ethanol; in large-scale studies, displays abnormal G2 phase progression and is haploinsufficient
Classical Genetics
- null
- conditional
- unspecified
- null
- repressible
- reduction of function
- conditional
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rfc4p interacts physically with proteins involved in DNA repair; RFC4 interacts genetically with genes involved in DNA repair
507 total interactions for 357 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
No regulation data available.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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