CIN5 / YOR028C Overview
- Standard Name
- CIN5 1
- Systematic Name
- YOR028C
- SGD ID
- SGD:S000005554
- Aliases
- HAL6 2 , YAP4 8
- Feature Type
- ORF , Verified
- Description
- Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication 2 3 4 5 6
- Name Description
- Chromosome INstability 7
- Paralog
- YAP6 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CIN5/YOR028C is located on the right arm of chromosome XV between Hsp90 cochaperone STI1 and YOR029W; coding sequence is 888 nucleotides long with 9 SNPs, 6 of which cause amino acid polymorphisms; alternative reference strain CEN.PK has a deletion at nucleotides 384420..384324; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cin5p is 295 amino acids long, low in abundance and very short-lived; sumoylated on K52 and phosphorylated on 15 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CIN5 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-binding transcription factor; involved in responses to hyperosmotic stress and xenobiotic stimuli
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor binding (IDA)
- enables sequence-specific DNA binding (IDA)
- High-Throughput
- enables sequence-specific DNA binding (HDA)
Biological Process
- Manually Curated
- involved in hyperosmotic response (IGI)
- involved in hyperosmotic salinity response (IMP)
- involved in regulation of transcription by RNA polymerase II (ISA)
- involved in response to xenobiotic stimulus (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; overexpression elevates resistance to MMS, mitomycin C and cisplatin; in systematic studies null mutants show increased competitive fitness and increased accumulation of alpha amino acids; the null mutant of paralog yap6 is viable; the cin5 yap6 double mutant has not been annotated for phenotype
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cin5p interacts physically with proteins involved in mitosis; CIN5 interacts genetically with genes involved in transcription by RNA polymerase II
150 total interactions for 136 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CIN5 encodes an AP-1 type transcription factor which contains a basic leucine zipper domain (bZIP), and binds to a consensus cis-acting Yap recognition element (YRE; 5'-TTAC/GTAA-3'). Cin5p is involved in the regulation of the osmotic stress response, and binds the promoters of dozens of genes, including those involved in the transport and catabolism of sugars and alcohol, and synthesis and accumulation of glycerol and trehalose. Cin5p also binds the promoters of over 50 different transcription factors, including its own. Cin5p is constitutively nuclear, and is induced upon exposure to various stress conditions, including mild hyperosmotic and oxidative stress, temperature shift or metal exposure. The CIN5 promoter contains several stress-associated cis-elements, including a YRE, four stress response elements (STRE), and a heat shock element (HSE). Expression of CIN5 is regulated by an interplay between the transcription factors Yap1p and Msn2p. Cin5p is phosphorylated, a modification that is driven by PKA and GSK3, and which stabilizes the protein. Cin5p nuclear localization is independent of its phosphorylation state, and activity is maintained in the absence of phosphorylation.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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