SPT23 / YKL020C Overview
- Standard Name
- SPT23 1
- Systematic Name
- YKL020C
- SGD ID
- SGD:S000001503
- Feature Type
- ORF , Verified
- Description
- ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication 2 3 4
- Name Description
- SuPpressor of Ty 1
- Paralog
- MGA2 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SPT23/YKL020C is located on the left arm of chromosome XI between alpha subunit of farnesyltransferase and geranylgeranyltransferase-I RAM2 and protein involved in 60S ribosomal subunit biogenesis MAK11; coding sequence is 3249 nucleotides long with 31 SNPs, 9 of which cause amino acid polymorphisms; alternative reference strain Sigma1278b has a deletion at nucleotides 398840..398832; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Spt23p is 1082 amino acids long and extremely low in abundance; phosphorylated on 24 residues and ubiquitinylated on 3 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPT23 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein involved in heterochromatin formation and positive regulation of transcription by RNA polymerase II; localized to the nucleus and endoplasmic reticulum membrane
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in cellular response to cold (IGI)
- involved in fatty acid homeostasis (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IGI, IMP)
- involved in positive regulation of unsaturated fatty acid biosynthetic process (IDA, IGI)
- involved in silent mating-type cassette heterochromatin formation (IGI)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum membrane (IDA)
- located in nucleus (IDA)
- High-Throughput
- located in endoplasmic reticulum (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant has decreased ergosterol levels and enlarged vacuoles with autophagic tubes that are suppressed by ergosterol supplementation; null mutant shows decreased cold shock tolerance and decreased resistance to camptothecin, mycophenolic acid, 4-chlorophenol, and visible light stress but increased resistance to elisidepsin, cycloheximide, fenpropimorph, and bleomycin; null mutant has increased competitive fitness in minimal medium and with ethanol, and increased replicative lifespan; overexpression causes abnormal budding, cell size, and morphology with decreased growth and viability, and decreased resistance to copper, manganese, hydroxyurea, radicicol, and fluconazole; is haploproficient under turbidostat growth conditions; the null mutant of paralog mga2 is viable; the spt23 mga2 double mutant is inviable
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Spt23p interacts physically with proteins involved in RNA catabolism; SPT23 interacts genetically with genes involved in transcription by RNA polymerase II
247 total interactions for 146 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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