UFD2 / YDL190C Overview
- Standard Name
- UFD2 1
- Systematic Name
- YDL190C
- SGD ID
- SGD:S000002349
- Feature Type
- ORF , Verified
- EC Number
- 2.3.2.27
- Description
- E4 ubiquitin ligase that extends E3-dependent Ub chains; catalyzes K48-linked multi-monoubiquitination of Ufd4p-assembled, K29-linked Ub chains prior to substrate degradation; binds and converts C-terminally extended Ub (Ubi4p) into an unanchored chain to mark client proteins for stress-induced degradation; role in Ub-fusion degradation, the OLE pathway, and the ERAD pathway; U-box family member; human homologs, UBE4A and UBE4B, implicated in neurological disease and tumor development 1 2 3 4 5 6 7 8 9 10 11
- Name Description
- Ubiquitin Fusion Degradation 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ufd2p is 961 amino acids long, low in abundance and average half-life; phosphorylated on S547 and ubiquitinylated on 12 residues
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all UFD2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-ubiquitin ligase (E4) involved in the ubiquitin-dependent ERAD pathway for protein catabolism; catalyses the K48-linked ubiquitination of proteins destined for degradation; localizes to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin-ubiquitin ligase activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to ethanol (IDA)
- involved in ERAD pathway (IMP)
- involved in positive regulation of protein ubiquitination (IMP)
- involved in positive regulation of reciprocal meiotic recombination (IMP)
- involved in proteasome-mediated ubiquitin-dependent protein catabolic process (IMP)
- involved in protein K48-linked ubiquitination (IDA)
- involved in protein ubiquitination (IDA)
- involved in ubiquitin-dependent protein catabolic process (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in reference strain S288C; null mutant requires myo-inositol for growth and has chromosome instability but decreased colony sectoring; increased chronological lifespan and decreased competitive fitness; decreased vegetative growth rate and sporulation; defective protein ubiquitination with stabilization of Mps1p; broad chemical sensitivity including DNA-damaging agents, protein synthesis inhibitors, and various other compounds but increased resistance to some DNA replication inhibitors and ER stress agents; increased cell surface metal reductase activity; small vacuolar fragmentation defects; is haploproficient; overexpression decreases vegetative growth rate; in unidentified strains, shows decreased Huntington toxin resistance
Classical Genetics
- null
- acid pH resistance: decreased
- auxotrophy
- chromosome/plasmid maintenance: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- haploproficient
- heat sensitivity: decreased
- heat sensitivity: increased
- metal resistance: decreased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation efficiency: decreased
- sporulation: decreased
- utilization of carbon source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Ufd2p interacts physically with proteins involved in response to chemical; UFD2 interacts genetically with genes involved in transcription by RNA polymerase II
377 total interactions for 242 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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