MSP1 / YGR028W Overview
- Standard Name
- MSP1 1
- Systematic Name
- YGR028W
- SGD ID
- SGD:S000003260
- Aliases
- YTA4 2
- Feature Type
- ORF , Verified
- Description
- Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; ATP-driven extractase that pulls mislocalized tail-anchored proteins into the cytosol, passing them to the GET pathway for transfer to the ER membrane for degradation, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain 1 2 3 4 5 6 7
- Name Description
- Mitochondrial Sorting of Proteins 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Highly-conserved N-terminally anchored AAA-ATPase that is 362 amino acids long, low in abundance and short lived; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain; ubiquitinylated at K42, phosphorylated at S40, S106, and S358
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MSP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATPase and dislocase involved in protein-mitochondrial targeting; role in the RADAR pathway involved in the degradation of peroxisomal matrix protein receptors in the absence of recyling; localizes to peroxisomal and mitochondrial outer membrane
View computational annotations
Molecular Function
- Manually Curated
- enables ATP hydrolysis activity (ISS)
- enables ATP-dependent peptidase activity (IDA)
- enables membrane protein dislocase activity (IMP)
Biological Process
- Manually Curated
- involved in extraction of mislocalized protein from mitochondrial outer membrane (IMP)
- involved in protein localization to mitochondrion (IMP)
- involved in protein targeting to ER (IDA)
- involved in RADAR pathway (IMP, IGI)
Cellular Component
- Manually Curated
- located in mitochondrial outer membrane (IDA)
- located in peroxisomal membrane (IDA)
- High-Throughput
- located in mitochondrial outer membrane (HDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has abnormal protein distribution with peroxisomal protein accumulation on mitochondria and increased Slt2p phosphorylation; in large-scale studies, null mutant displays variable competitive fitness depending on conditions, increased chronological and replicative lifespans, increased thermotolerance, haploinsufficiency, increased metal resistance to gadolinium, and abnormal vacuolar morphology; overexpression increases invasive growth and decreases vegetative growth rate
Classical Genetics
- null
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- innate thermotolerance: increased
- metal resistance: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Msp1p interacts physically with proteins involved in protein folding; MSP1 interacts genetically with genes involved in DNA replication
205 total interactions for 151 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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