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OPI3 / YJR073C Overview

Standard Name
OPI3 1
Systematic Name
YJR073C
SGD ID
SGD:S000003834
Aliases
PEM2 2 4
Feature Type
ORF , Verified
EC Number
2.1.1.17, 2.1.1.71
Description
Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase 2 3
Name Description
OverProducer of Inositol 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Summary
OPI3/YJR073C is located on the right arm of chromosome X beteen NPA3 GTPase and MOG1; coding sequence is 621 nucleotides long with 5 SNPs, 1 of which causes an amino acid polymorphism

Analyze Sequence

S288C only

BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation

S288C vs. other species

BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser

S288C vs. other strains

Strain Alignment | Variant Viewer

Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Summary
Opi3p is 206 amino acids long, low in abundance
AlphaFold predicted structure of OPI3
EC Number
2.1.1.17, 2.1.1.71
Length (a.a.)
206
Mol. Weight (Da)
23156.6
Isoelectric Point
9.26
Median Abundance (molecules/cell)
7713 +/- 4122

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all OPI3 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Summary
Phosphatidyl-N-methylethanolamine N-methyltransferase involved in phosphatidylcholine biosynthesis; putative integral membrane protein

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
High-Throughput

Metabolic Pathways

Metabolic Pathways annotations describe biochemical pathways involving this gene product in small molecule metabolism. These annotations connect gene products to specific metabolic reactions, substrates, and products within larger metabolic networks. Metabolic pathway data is curated from published scientific literature. Click the links to see detailed pathway diagrams on SGD's YeastPathways site for further exploration.

Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Summary
Non-essential gene in reference strain S288C; null mutant displays Opi- phenotype (overproduction and excretion of inositol in the absence of inositol and choline), abnormal ER morphology, and reduced resistance to desiccation; in large-scale studies null mutant shows reduced competitive fitness, decreased innate thermotolerance, increased glycogen accumulation, reduced filamentous growth, and sensitivity to various chemicals

Classical Genetics

null

Large-scale Survey

null
overexpression
conditional
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Summary
Opi3p interacts physically with proteins involved in mitotic cell cycle; OPI3 interacts genetically with genes involved in lipid metabolism

1388 total interactions for 623 unique genes

Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Summary
OPI3/YJR073C transcription is positively regulated by Gcr1p during lipid homeostasis, negatively regulated by Pah1p during nuclear membrane biogenesis, regulated by Ino2p in response to glucose starvation, and by Bur6p, Fkh1p, Med2p, Med4p, Sua7p, and Xbp1p in response to heat.
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2026-02-26

Literature Details

All Curated Literature

All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).

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Resources

AlphaFold Protein Structure | E.C.2.1.1.17 | E.C.2.1.1.71 | Entrez Gene | Entrez RefSeq Protein | FungiDB | Search all NCBI | UniParc | UniProtKB