SAM1 / YLR180W Overview

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

EC Number

2.5.1.6

Length (a.a.)

382

Mol. Weight (Da)

41803.6

Isoelectric Point

4.83

Median Abundance (molecules/cell)

65133 +/- 31585

Half-life (hr)

8.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Functional Networks

Functional Networks display how gene products work together in biological systems. The Shared Annotations network shows genes with similar GO annotations, suggesting functional relationships. GO-CAMs (Gene Ontology Causal Activity Models) are manually curated pathway models that illustrate how molecular activities of multiple gene products connect through causal relationships to carry out biological processes. GO-CAMs integrate Molecular Function, Biological Process, and Cellular Component information into unified pathway representations based on published experimental evidence. Click "View GO-CAM at Gene Ontology" to explore the interactive model at AmiGO.

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Metabolic Pathways

Metabolic Pathways annotations describe biochemical pathways involving this gene product in small molecule metabolism. These annotations connect gene products to specific metabolic reactions, substrates, and products within larger metabolic networks. Metabolic pathway data is curated from published scientific literature. Click the links to see detailed pathway diagrams on SGD's YeastPathways site for further exploration.

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Summary

Non-essential gene in reference strain S288C; null mutant has increased replicative lifespan; decreased competitive fitness and growth rate; haploinsufficient under turbidostat growth conditions; increased heat sensitivity; in a non-S288C strain, the heterozygous diploid null mutant has increased resistance to hydroxyurea, benomyl, and phleomycin; in W303, the homozygous diploid null mutant has altered nucleotide metabolism with decreased dTTP and glutathione, increased dATP and dCTP; in large-scale studies, the null mutant in S288C displays abnormal amino acid profile with increased methionine, defect in salt-induced vacuolar fragmentation, and sensitivity to various chemicals including sinefungin, chitosan, and tellurite; the sam1 null mutant is viable; the null mutant of paralog sam2 is viable; the sam1 sam2 double mutant is inviable or displays a growth defect

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

All Curated Literature

All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).

Resources