PHO84 / YML123C Overview
- Standard Name
- PHO84
- Systematic Name
- YML123C
- SGD ID
- SGD:S000004592
- Aliases
- phoT 9
- Feature Type
- ORF , Verified
- Description
- High-affinity inorganic phosphate (Pi) transporter and sensor; transceptor that acts as a phosphate sensor for rapid phosphate signaling; involved in inositol pyrophosphate-regulated myo-inositol export; low-affinity manganese and selenite transporter; regulated by Pho4p and Spt7p; exit from the ER during maturation requires Pho86p; overexpressor accumulates heavy metals but does not develop metal toxicity; human homolog SLC2A2, also involved in inositol export, complements a pho84 opi1 null 1 2 3 4 5 6 7 8
- Name Description
- PHOsphate metabolism
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- PHO84/YML123C is located on the left arm of chromosome XIII near the telomere between TUB3 alpha tubulin and uncharacterized gene YML122C; coding sequence is 1764 nucleotides long with 24 SNPs, 6 of which cause amino acid polymorphisms, and an in-frame 3-nucleotide deletion in strains SEY6210 and FL100 that removes residue Ile309
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pho84p is 587 amino acids long, low in abundance; contains disordered region at C-terminus and two sugar transporter conserved sites
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PHO84 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Inorganic phosphate transmembrane transporter involved in phosphate ion transport, polyphosphate metabolism and phosphate sensing; contains manganese ion transmembrane transporter activity and selenite:proton symporter activity involved in the transport of selenite across the plasma membrane; involved in myo-inositol export; integral component of the plasma membrane; also localizes to the vacuole lumen and membrane
View computational annotations
Molecular Function
- Manually Curated
- enables manganese ion transmembrane transporter activity (IMP)
- enables phosphate transmembrane transporter activity (IMP, IDA)
- enables selenite:proton symporter activity (IMP, IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in manganese ion transport (IMP)
- involved in phosphate ion transport (IMP, IDA)
- involved in plasma membrane selenite transport (IMP)
- involved in polyphosphate metabolic process (IMP)
Cellular Component
- Manually Curated
- located in plasma membrane (IDA)
- High-Throughput
- located in endoplasmic reticulum (HDA)
- located in fungal-type vacuole (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- PHO84/YML123C is a non-essential gene; null mutant is viable, fails to grow in low-phosphate medium, does not take up inorganic phosphate, fails to accumulate polyphosphate, has reduced competitive fitness in a variety of media, and altered resistance to various chemicals; ts mutant has elevated acid phosphatase activity at elevated temperature in the presence of inorganic phosphate
Classical Genetics
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- growth in exponential phase: decreased rate
- growth in exponential phase: increased rate
- lipid particle morphology: abnormal
- metal resistance: increased
- nutrient uptake: absent
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- small molecule transport: absent
- small molecule transport: decreased rate
- vegetative growth: absent
- vegetative growth: increased
- conditional
- misexpression
- null
- biofilm formation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- haploinsufficient
- heat sensitivity: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- utilization of nitrogen source: decreased rate
- utilization of phosphorus source: decreased
- vegetative growth: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pho84p interacts physically with proteins involved in mitotic cell cycle and organelle fission; PHO84 interacts genetically with genes involved in transcription
297 total interactions for 240 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-06-11
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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