PHO23 / YNL097C Overview
- Standard Name
- PHO23 1
- Systematic Name
- YNL097C
- SGD ID
- SGD:S000005041
- Feature Type
- ORF , Verified
- Description
- Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3 1 2 3 4 5 6
- Name Description
- PHOsphate metabolism 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | CGD Synteny Browser
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pho23p is 330 amino acids long, low in abundance and slightly shorter-lived; phosphorylated on 10 residues, sumoylated on 4 residues, and monoacetylated on K60, K154, and K183
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PHO23 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Histone-binding component of the Rpd3L histone deacetylase complex involved in chromatin organization, modification, and silencing at telomeres, the rDNA, and the mating-type cassette; regulates transcription in response to stressors such as heat and glucose limitation
View computational annotations
Molecular Function
- Manually Curated
- enables histone H3K4me3 reader activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in cellular response to heat (IMP)
- involved in chromatin organization (IMP)
- involved in negative regulation of rDNA heterochromatin formation (IMP)
- involved in negative regulation of silent mating-type cassette heterochromatin formation (IMP)
- involved in negative regulation of transcription by RNA polymerase I (IMP)
- involved in positive regulation of invasive growth in response to glucose limitation (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
Cellular Component
- Manually Curated
- part of histone deacetylase complex (IDA)
- part of Rpd3L complex (IDA)
- High-Throughput
- located in nucleus (HDA)
- part of Rpd3L complex (HDA)
- part of Rpd3L-Expanded complex (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- PHO23/YNL097C is a non-essential gene in reference strain S288C; null mutants are viable but grow slowly and display haploinsufficiency, decreased utilization of carbon and nitrogen sources, decreased respiratory growth rate, decreased filamentous growth, and absent invasive growth. Null mutants exhibit abnormal chemical compound accumulation, increased chemical compound excretion, abnormal protein/peptide distribution, increased protein/peptide modification, abnormal and increased RNA accumulation, abnormal vacuolar morphology, decreased vacuolar transport, and decreased endocytosis. Stress resistance phenotypes are context-dependent: null mutants show decreased innate thermotolerance, increased heat sensitivity, decreased desiccation resistance, decreased starvation resistance, decreased overall stress resistance, decreased resistance to toxins, and variable resistance to chemicals (both increased and decreased depending on the compound), while metal resistance and oxidative stress resistance are increased. Null mutants display increased silencing, decreased mutation frequency, decreased transposable element transposition, decreased replicative lifespan, increased sporulation efficiency, and decreased competitive fitness. Overexpression of PHO23 results in decreased or normal vegetative growth rate (context-dependent) and increased invasive growth. Reduction-of-function alleles exhibit increased acid pH resistance, increased osmotic stress resistance, and variable resistance to metals and chemicals (both increased and decreased depending on the specific stressor).
Classical Genetics
- null
- overexpression
- null
- chemical compound accumulation: abnormal
- chemical compound excretion: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- filamentous growth: decreased
- haploinsufficient
- heat sensitivity: increased
- invasive growth: absent
- metal resistance: increased
- mutation frequency: decreased
- oxidative stress resistance: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- sporulation efficiency: increased
- starvation resistance: decreased
- stress resistance: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pho23p interacts physically with proteins involved in transcription by RNA polymerase II; PHO23 interacts genetically with genes involved in transcription by RNA polymerase II
1701 total interactions for 856 unique genes
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
All Curated Literature
All manually curated literature for the specified gene, shown as a count of references by year of publication followed by the most recent papers. Click "Literature Details" or "See all" to view all literature information for this locus, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review).
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